Description

Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. Expression vectors with Glutathione S-transferase (GST) tag have beed developed widely for both prokaryotic and eukaryotic systems, because GST can provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein encoded by inserted cDNA sequences. Antibodies of anti-GST tag are useful for checking protein expression using immunoblotting, immunoprecipitation and immunostaining techniques, as well as for immunoaffinity purification of protein expressed in vitro.

References
1. 1993. Biotechnology (N Y) 11: 933-6. 2. 1988. Gene 67: 31-40.

Specification
clone	6G9C6
Host/Isotype	Mouse IgG1
Antibody Type	Tag Antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Tag
Immunogen	Purified fusion protein with GST protein tag expressed in E. Coli.
Formulation	Purified antibody in PBS containing 0.03% sodium azide.

Application
WB	1/500 - 1/2000
IP	1/20
IHC	1/100 - 1/1000
ICC	1/100 - 1/1000
FCM	1/200 - 1/400
ELISA	1/10000