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Mouse Monoclonal Antibody to ALCAM
Description |
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This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. |
References |
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1. Vascul Pharmacol. 2011 Mar-Jun;54(3-6):93-9. 2. Int J Gynecol Cancer. 2011 Apr;21(3):523-8. |
Specification | |
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Aliases | MEMD; CD166; FLJ38514; MGC71733 |
Entrez GeneID | 214 |
Swissprot | Q13740 |
clone | 8E12C7 |
WB Predicted band size | 65.1kDa |
Host/Isotype | Mouse IgG2a |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Purified recombinant fragment of human ALCAM (AA: 48-216) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application | |
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IHC | 1/200 - 1/1000 |
ICC | 1/200 - 1/1000 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
- Immunofluorescence analysis of Hela cells using ALCAM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
- Flow cytometric analysis of JURKAT cells using ALCAM mouse mAb (green) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using ALCAM mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using ALCAM mouse mAb with DAB staining.
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30512
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