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Rabbit Polyclonal Antibody to DANRE atg4b (N-term)
Description |
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Cysteine protease required for autophagy, which is able to cleave the C-terminal part of proteins that may be subsequently converted to a smaller form, with a revealed C-terminal glycine, considered to be the phosphatidylethanolamine (PE)-conjugated form. This conjugated form has the capacity for the binding to autophagosomes (By similarity). |
Specification | |
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Aliases | Cysteine protease ATG4B, 3422-, Autophagy-related protein 4 homolog B, atg4b, apg4b |
Swissprot | Q6DG88 |
WB Predicted band size | 44.5kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse,zebrafish, |
Immunogen | This DANRE atg4b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of DANRE atg4b. |
Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Application | |
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WB | 1/1000 |
Product Image
- All lanes : Anti-(DANRE) atg4b Antibody (N-term) at 1:1000 dilution Lane 1: Zebrafish lysate Lane 2: ZF4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
- DANRE atg4b Antibody (N-term) (Cat. #P34232) western blot analysis in zebra fish brain tissue lysates (35ug/lane).This demonstrates the DANRE atg4b antibody detected the DANRE atg4b protein (arrow).
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#P34232
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