Description

IGF1R (insulin-like growth factor 1 receptor), a transmembrane receptor tyrosine kinase, is widely expressed in many cell types within fetal and postnatal tissues, and in many cell lines. Upon binding to its ligands, IGF-I and IGF-II, receptor autophosphorylation occurs. The triple tyrosine cluster within the kinase domain (Tyr1131, Tyr1135 and Tyr1136) is the earliest major site of autophosphorylation. Phosphorylation of these three tyrosine residues is necessary for kinase activation.Insulin receptors (IRs) share significant similarity with IGF1 receptors in both structure and function,including an equivalent triple tyrosine cluster within the activation loop of the kinase domain (Tyr1146, Tyr1150 and Tyr1151).Tyrosine autophosphorylation of insulin receptor is one of the earliest cellular responses to insulin stimulation. Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151. Full kinase activation requires the triple tyrosine phosphorylation.

References
1. Adams, T.E. et al. Cell. Mol. Life Sci. 2000 57, 1050-1093. 2. Baserga, R. et al. Oncogene 2000 19, 5574-5581. 3. Scheidegger, K.J. et al. J. Biol. Chem. 2000 275, 38921-38928.

Specification
Aliases	IGF1R, IGF1R-Beta
Entrez GeneID	3480
Swissprot	P08069
clone	3C8B1
WB Predicted band size	96kDa
Host/Isotype	Mouse IgG2b
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Purified recombinant fragment of IGF1R-Beta expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Application
IHC	1/200 - 1/1000
ELISA	1/10000