Description

GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

References
1. Proc Natl Acad Sci U S A. 2006, Jul 25,103(30):11399-404. 2. Gene Expr Patterns. 2007, Jan,7(1-2):124-30. 3. Eur J Cell Biol. 2006, Sep,85(9-10):1059-68.

Specification
Swissprot	P42212
clone	4B10B2
WB Predicted band size	27kDa
Host/Isotype	Mouse IgG2a
Antibody Type	Tag Antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Tag
Immunogen	Purified recombinant fragment of GFP expressed in E. Coli.
Formulation	Purified antibody in PBS containing 0.03% sodium azide.

Application
WB	1/500-1/1000
IHC	1/200 - 1/1000
ICC	1/100-1/200
FCM	1/200 - 1/400
ELISA	1/10000