Description

Mcl-1 (Myeloid cell leukemia-1) is Bcl-2-related and was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-alpha 1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.This monoclonal antibody detected a 37kd MCL1 in BCBL-1 cell lysate.

References
1. Ota, N. et al. J. Hum. Genet. 2000. 46: 254-269. 2. Schwertfeger KL, Ryder JW, Anderson SM J Mammary Gland Biol Neoplasia 2000, 3 : 236-251.

Specification
Aliases	EAT, MCL1L, MCL1S
Entrez GeneID	4170
Swissprot	Q07820
clone	8C6D4B1
WB Predicted band size	37kDa
Host/Isotype	Mouse IgG1
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Purified recombinant fragment of human MCL-1 expressed in E. Coli.
Formulation	Purified antibody in PBS containing 0.03% sodium azide.

Application
WB	1/500 - 1/2000
IHC	1/200 - 1/1000
ICC	1/200 - 1/1000
ELISA	1/10000