Description

Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1L2 or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation.

References
1. Cell Cycle. 2006 Nov 1;5(21):2537-42. 2. FEBS Lett. 2007 Jun 26;581(16):3005-12.

Specification
Aliases	SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; KIAA0178; MGC138332; SMC1alpha; DKFZp686L19178; SMC1A
Entrez GeneID	8243
Swissprot	Q14683
clone	5B6
WB Predicted band size	143kDa
Host/Isotype	Mouse IgG1
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Purified recombinant fragment of human SMC1 expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Application
WB	1/500 - 1/2000
IHC	1/200 - 1/1000
ICC	1/200 - 1/1000
FCM	1/200 - 1/400
ELISA	1/10000