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Mouse Monoclonal Antibody to TWIST1
Description |
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Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. |
References |
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1. Cancer Res. 2013 Jan 15;73(2):662-71. 2. Cancer Res. 2012 Dec 15;72(24):6382-92. |
Specification | |
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Aliases | SCS; ACS3; CRS1; BPES2; BPES3; TWIST; bHLHa38 |
Entrez GeneID | 7291 |
Swissprot | Q15672 |
clone | 10E4E6 |
WB Predicted band size | 21kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse |
Immunogen | Purified recombinant fragment of human TWIST1 (AA: 9-74) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application | |
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WB | 1/500 - 1/2000 |
IHC | 1/200 - 1/1000 |
ICC | 1/200 - 1/1000 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
- Western blot analysis using TWIST1 mouse mAb against NIH/3T3 (1), JURKAT (2), HELA (3), A549 (4), RAJI (5) and OCM-1 (6) cell lysate.
- Immunofluorescence analysis of Hela cells using TWIST1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
- Flow cytometric analysis of Hela cells using TWIST1 mouse mAb (green) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using TWIST1 mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using TWIST1 mouse mAb with DAB staining.
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30506
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