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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using TWIST1 mouse mAb against NIH/3T3 (1), JURKAT (2), HELA (3), A549 (4), RAJI (5) and OCM-1 (6) cell lysate.
Immunofluorescence analysis of Hela cells using TWIST1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric analysis of Hela cells using TWIST1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using TWIST1 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using TWIST1 mouse mAb with DAB staining.

Mouse Monoclonal Antibody to TWIST1

  • 货号:

    30506
  • 别名:

    SCS; ACS3; CRS1; BPES2; BPES3; TWIST; bHLHa38
  • 应用:

    WB,IHC,ICC,FCM
  • 反应种属:

    Human,Mouse
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to TWIST1

Description
Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome.


References
1. Cancer Res. 2013 Jan 15;73(2):662-71.
2. Cancer Res. 2012 Dec 15;72(24):6382-92.
Specification
Aliases SCS; ACS3; CRS1; BPES2; BPES3; TWIST; bHLHa38
Entrez GeneID 7291
Swissprot Q15672
clone 10E4E6
WB Predicted band size 21kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse
Immunogen Purified recombinant fragment of human TWIST1 (AA: 9-74) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application
WB 1/500 - 1/2000
IHC 1/200 - 1/1000
ICC 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000