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Mouse Monoclonal Antibody to CFLAR
Description |
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The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. |
References |
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1. PLoS One. 2012;7(9):e44917. 2. Cancer Prev Res (Phila). 2012 Apr;5(4):612-20. |
Specification | |
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Aliases | CASH; FLIP; MRIT; CLARP; FLAME; Casper; FLAME1; c-FLIP; FLAME-1; I-FLICE; c-FLIPL; c-FLIPR; c-FLIPS; CASP8AP1 |
Entrez GeneID | 8837 |
Swissprot | O15519 |
clone | 7A3D12 |
WB Predicted band size | 55kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse |
Immunogen | Purified recombinant fragment of human CFLAR (AA: 100-251) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application | |
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WB | 1/500 - 1/2000 |
IHC | 1/200 - 1/1000 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
- Western blot analysis using CFLAR mouse mAb against JURKAT (1), 3T3L1 (2) and RAJI (3) cell lysate.
- Flow cytometric analysis of JURKAT cells using CFLAR mouse mAb (green) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using CFLAR mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded human esophagus cancer tissues using CFLAR mouse mAb with DAB staining.
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30511
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