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Mouse Monoclonal Antibody to TFAP2A
Description |
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The protein encoded by this gene is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. Defects in this gene are a cause of branchiooculofacial syndrome (BOFS). Three transcript variants encoding different isoforms have been found for this gene. |
References |
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Mol Hum Reprod. 2011 Nov;17(11):702-9. Breast Cancer Res. 2011 Mar 4;13(2):R23. |
Specification | |
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Aliases | AP-2; BOFS; AP2TF; TFAP2; AP-2alpha |
Entrez GeneID | 7020 |
Swissprot | P05549 |
clone | 1A10C5 |
WB Predicted band size | 48kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Purified recombinant fragment of human TFAP2A (AA: 1-100) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application | |
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ICC | 1/200 - 1/1000 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
- Immunofluorescence analysis of Hela cells using TFAP2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
- Flow cytometric analysis of Hela cells using TFAP2A mouse mAb (green) and negative control (red).
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30770
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