Description

Glycogen synthase kinase 3 alpha belongs to the Ser/Thr family of protein kinases, Cdc2/cdkx subfamily,GSK3 subsubfamily. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, myb, and the transcription factor c jun. GSK3 phosphorylates glycogen synthase and thereby inactivates it. Insulin stimulates the dephosphorylation of glycogen synthase at the sites phosphorylated by GSK3 and subsequently inhibits GSK3 acutely leading to the stimulation of glycogen synthesis. GSK3 signaling is performed by two isoforms, GSK3 alpha and GSK3 beta. The two isoforms share 97% sequence similarity within their catalytic domains. GSK3 has also been shown to play a role in protein synthesis, cell adhesion, cell proliferation, cell differentiation, microtubule dynamics and cell motility.

References
1. Mendez P, Garcia-Segura LM. Endocrinology. 2006 Jun;147(6):3027-39. Epub 2006 Feb 23. 2. Bianchi M, De Lucchini S, et, al. Biochem J. 2005 Oct 15;391(Pt 2):359-70. 3. De Servi B, Hermani A, Medunjanin S, Mayer D. Oncogene. 2005 Jul 21;24(31):4946-55.

Specification
Aliases	DKFZp686D0638; GSK3A
Entrez GeneID	2931
Swissprot	P49840
clone	6G12C2
Host/Isotype	Mouse IgG1
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Purified recombinant fragment of GSK3 alpha expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Application
WB	1/500 - 1/2000
ELISA	1/10000