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Mouse Monoclonal Antibody to PLD2
Description |
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The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene. |
References |
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1.Exp Mol Med. 2014 Dec 5;46:e124. 2.FEBS Lett. 2014 Aug 25;588(17):3251-8. |
Specification | |
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Entrez GeneID | 5338 |
Swissprot | O14939 |
clone | 7E4D9 |
WB Predicted band size | 106kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Purified recombinant fragment of human PLD2 (AA: 834-933) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application | |
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IHC | 1/200 - 1/1000 |
ICC | 1/200 - 1/1000 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
- Immunofluorescence analysis of MCF-7 cells using PLD2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
- Immunofluorescence analysis of SK-OV-3 cells using PLD2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
- Flow cytometric analysis of Hela cells using PLD2 mouse mAb (green) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human renal tissues using PLD2 mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using PLD2 mouse mAb with DAB staining.
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#31024
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