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Mouse Monoclonal Antibody to CD2
Description |
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CD2 is a surface antigen of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells (summarized by Sewell et al., 1986 [PubMed 3490670]). It is one of the earliest T-cell markers, being present on more than 95% of thymocytes; it is also found on some natural killer cells but not on B lymphocytes. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it. |
References |
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1.J Virol. 2013 Aug;87(16):9148-58. 2.PLoS One. 2012;7(10):e47664. |
Specification | |
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Aliases | T11; SRBC; LFA-2 |
Entrez GeneID | 914 |
Swissprot | P06729 |
clone | 3D1E3 |
WB Predicted band size | 39.4kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Mouse |
Immunogen | Purified recombinant fragment of human CD2 (AA: 25-140) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application | |
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WB | 1/500 - 1/2000 |
IHC | 1/200 - 1/1000 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
- Western blot analysis using CD2 mouse mAb against MOLT4 (1), MCF-7 (2), L1210 (3), U937 (4), and NIH3T3 (5) cell lysate.
- Flow cytometric analysis of Hela cells using CD2 mouse mAb (green) and negative control (red).
- Flow cytometric analysis of HepG2 cells using CD2 mouse mAb (green) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using CD2 mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using CD2 mouse mAb with DAB staining.
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#31026
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