His Tag[32442]

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Red: Control Antigen (100ng) Purple: Antigen (10ng), Green: Antigen (50ng) Blue: Antigen (100ng),

Western blot analysis using His tag mAb against recombinant protein. lane 1 :(100 ng); lane 2 :(50 ng); lane 3 :(25 ng); lane 4 :(10 ng); lane 5 :(2.5 ng); (Expected MW is 50 kDa).

HOT

Mouse Monoclonal Antibody to His Tag

货号：
32442
别名：
6 His tag; HHHHHH tag
应用：
WB,IP,IHC,ICC,FCM
反应种属：
Tag
规格：

100μl
50μl
数量：

-

+
说明书：

目录价¥1320

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Mouse Monoclonal Antibody to His Tag

Description
The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.

Description

The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.

Specification
Aliases	6 His tag; HHHHHH tag
clone	6A8H8
Host/Isotype	Mouse IgG1
Antibody Type	Tag Antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Tag
Immunogen	Synthesized peptide of His Tag (AA: HHHHHHHH-KLH).
Formulation	Purified antibody in PBS with 0.05% sodium azide