6×His Tag[P00002]

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Gel: 8%SDS-PAGE, Lysate: 1/0.5/0.5 μg, Lane 1-3: Two fusion protein containing the HIS Tag, Primary antibody: P00002(6×His Tag Antibody) at dilution 1/1000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 3 seconds

Rabbit Polyclonal Antibody to 6×His Tag

货号：
P00002
别名：
6 His epitope tag
应用：
WB
反应种属：
Fusion proteins containing the HIS Tag
规格：

100μl
50μl
数量：

-

+
说明书：

目录价¥1980

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Rabbit Polyclonal Antibody to 6×His Tag

Description
The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification. It can then be purified using immobilized metal ions, traditionally nickel, in a procedure termed immobilized-metal affinity chromatography. Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting. The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.

Description

The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification. It can then be purified using immobilized metal ions, traditionally nickel, in a procedure termed immobilized-metal affinity chromatography. Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting. The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.

Specification
Aliases	6 His epitope tag
WB Predicted band size	50/44 kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Fusion proteins containing the HIS Tag
Immunogen	Synthetic peptide: 18×His conjugated to KLH
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol