Description

DAXX (death-domain associated protein), it is a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants.

References
1. Cell. 1997 Jun 27;89(7):1067-76. 2. Biochem Biophys Res Commun. 2000 Dec 9;279(1):6-10. 3. Proc Natl Acad Sci U S A. 2004 Aug 17;101(33):12130-5.

Specification
Aliases	DAP6; EAP1; BING2
Entrez GeneID	1616
Swissprot	Q9UER7
clone	7A11
WB Predicted band size	81kDa
Host/Isotype	Mouse IgG1
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Purified recombinant fragment of human DAXX expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Application
WB	1/500 - 1/2000
ICC	1/200 - 1/1000
FCM	1/200 - 1/400
ELISA	1/10000