Description

This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.

References
1. J Biol Chem. 2004 Sep 10;279(37):38360-8. 2. Immunity. 2005 Aug;23(2):203-12. 3. J Clin Invest. 2010 Apr 1;120(4):1337-44. doi: 10.1172/JCI41305.

Specification
Aliases	RAG-2; RAG2
Entrez GeneID	5897
Swissprot	P55895
clone	4D5
WB Predicted band size	59kDa
Host/Isotype	Mouse IgG1
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Purified recombinant fragment of human RAG2(350-527aa) expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Application
ELISA	1/10000