Description

The cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.

References
1. Mol Biol Cell. 2008 Aug;19(8):3536-43. 2. Eur J Cancer. 2009 May;45(8):1466-73.

Specification
Aliases	CDC2; CDC28A; P34CDC2; MGC111195; DKFZp686L20222; CDK1
Entrez GeneID	983
Swissprot	P06493
clone	8C5A6
WB Predicted band size	34kDa
Host/Isotype	Mouse IgG1
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Purified recombinant fragment of CDC2 expressed in E. Coli.
Formulation	Ascitic fluid containing 0.03% sodium azide.

Application
WB	1/500 - 1/2000
IHC	1/200 - 1/1000
ELISA	1/10000