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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western blot analysis using SRC mouse mAb against MCF-7 (1), A431 (2), Hela (3), HEK293 (4), NIH/3T3 (5), PC-12 (6) and Cos7 (7) cell lysate.
Flow cytometric analysis of MCF-7 cells using SRC mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using SRC mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human esophageal tissues using SRC mouse mAb with DAB staining.
Mouse Monoclonal Antibody to SRC
| Description |
|---|
| This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. |
| References |
|---|
| 1. Clin Cancer Res. 2009 Nov 15;15(22):6852-61. 2. Cell. 2009 Nov 13;139(4):693-706. |
| Specification | |
|---|---|
| Aliases | ASV; SRC1; c-SRC; p60-Src |
| Entrez GeneID | 6714 |
| Swissprot | P12931 |
| clone | 1F11 |
| WB Predicted band size | 60kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human SRC expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
| Application | |
|---|---|
| WB | 1/500 - 1/2000 |
| IHC | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
- Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
- Western blot analysis using SRC mouse mAb against MCF-7 (1), A431 (2), Hela (3), HEK293 (4), NIH/3T3 (5), PC-12 (6) and Cos7 (7) cell lysate.
- Flow cytometric analysis of MCF-7 cells using SRC mouse mAb (green) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using SRC mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded human esophageal tissues using SRC mouse mAb with DAB staining.
For Reseach Only
Application Key：WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30290
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