Description

This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways.

References
1.Nucleic Acids Res. 2021 Apr 19;49(7):3948-3966. 2.Carcinogenesis. 2010 Jun;31(6):994-1002.

Specification
Aliases	RP-A p34, RPA2, REPA2, RPA32, RPA34
Entrez GeneID	6118
Swissprot	P15927
clone	2C5B6
WB Predicted band size	29kDa
Host/Isotype	Mouse IgG1
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Purified recombinant fragment of human RFA2 expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Application
WB	1/500 - 1/2000
IHC	1/200 - 1/1000
FCM	1/200 - 1/400