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Mouse Monoclonal Antibody to ULK3
Description |
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Enables protein serine/threonine kinase activity. Involved in several processes, including fibroblast activation; protein autophosphorylation; and regulation of smoothened signaling pathway. Located in cytoplasm. |
References |
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1.Biochem J. 2021 Jul 30;478(14):2811-2823. 2.Elife. 2015 May 26;4:e06547. |
Specification | |
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Entrez GeneID | 25989 |
Swissprot | Q6PHR2 |
clone | 5A5G2 |
WB Predicted band size | 53.4kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Rat |
Immunogen | Purified recombinant fragment of human ULK3 expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application | |
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WB | 1/500 - 1/2000 |
IHC | 1/200 - 1/1000 |
ICC | 1/50 - 1/200 |
FCM | 1/200 - 1/400 |
Product Image
- Western blot analysis using ULK3 mouse mAb against rat testis (1), PC-3 (2), DU145 (3),HEK293 (4),LNCAP (5)and Hela (6) cell lysate.
- Immunofluorescence analysis of Hela cells using ULK3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
- Flow cytometric analysis of HEK293 cells using ULK3 mouse mAb (green) and negative control (red).
- Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using ULK3 mouse mAb with DAB staining.
- Immunohistochemical analysis of paraffin-embedded human prostate cancer tissues using ULK3 mouse mAb with DAB staining.
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#32395
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