Description

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.

References
1.Biomaterials. 2019 Mar;197:72-85. 2.J Allergy Clin Immunol. 2021 Feb;147(2):622-632.

Specification
Aliases	TPS1; TPS2; TPSB1; TPSB2; Tryptase-2
Entrez GeneID	7177
Swissprot	Q15661
clone	6B6B12
WB Predicted band size	30.5kDa
Host/Isotype	Mouse IgG2b
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Rat
Immunogen	Purified recombinant fragment of human TPSAB1 (AA: 31-275) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Application
IHC	1/200 - 1/1000
FCM	1/200 - 1/400
ELISA	1/10000