Description

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family. A mutation in an intron of this gene is associated with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts. Alternative splicing results in multiple transcript variants.

References
1.Int J Mol Med. 2018 Nov;42(5):2923-2929. 2.Cancer Res. 2017 Dec 1;77(23):6627-6640.

Specification
Aliases	JAMC; JAM-2; JAM-3; JAM-C
Entrez GeneID	83700
Swissprot	Q9BX67
clone	2A5B6
WB Predicted band size	35kDa
Host/Isotype	Mouse IgG1
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Purified recombinant fragment of human JAM3 (AA: extra 32-241) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Application
WB	1/500 - 1/2000
FCM	1/200 - 1/400
ELISA	1/10000