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Mouse Monoclonal Antibody to GOT2
Description |
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Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. |
References |
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1. Hepatology. 1998 Apr;27(4):1064-74. 2. Cell. 2005 Sep 23;122(6):957-68. 3. Psychiatr Genet. 2007 Oct;17(5):314. |
Specification | |
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Aliases | KAT4; KATIV; mitAAT |
Entrez GeneID | 2806 |
Swissprot | P00505 |
clone | 3E9 |
WB Predicted band size | 47kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse,Rat,Monkey |
Immunogen | Purified recombinant fragment of human GOT2 expressed in E. Coli. |
Formulation | Ascitic fluid containing 0.03% sodium azide. |
Application | |
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WB | 1/500 - 1/2000 |
ICC | 1/200 - 1/1000 |
ELISA | 1/10000 |
Product Image
- Western blot analysis using GOT2 mouse mAb against HEK293 (1), PC-12 (2), HL-60 (3), BCBL-1 (4), HepG2 (5) and NIH/3T3 (6) cell lysate.
- Immunofluorescence analysis of PC-3 (left) and SK-BR-3 (right) cells using anti-GOT2 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
For Reseach Only
Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation
#30004
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