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Western blot analysis using beta-Actin mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), CHO (4), PC12 (5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysate.
Confocal Immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using beta Actin mouse mAb (red, the secondary Ab is Cy3-Goat anti mouse IgG). Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric analysis of MCF-7 cells using beta Actin mouse mAb (right) and negative control (left).
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Mouse Monoclonal Antibody to beta-Actin

  • 货号:

    20270
  • 别名:

    PS1TP5BP1; ACTB
  • 应用:

    WB,ICC,FCM
  • 反应种属:

    Human,Mouse,Hamster,Rat,Monkey,Dog
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥1320

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Mouse Monoclonal Antibody to beta-Actin

Description
Beta-actin (PS1TP5-binding protein 1), also known as ACTB, PS1TP5BP1. Entrez Protein NP_001092. It is one of six different actin proteins. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton.Actins are highly conserved proteins that are involved in various types of cell motility, structure, and integrity. Actin is ubiquitously expressed in all eukaryotic cells. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
References
1. Proteomics. 2005 Oct;5(15):3876-84.
2. PLoS Med. 2005 Oct;2(10):e263.
3. Mol Biol Cell. 2005 Nov;16(11):5055-60
4. Nature. 2005 Oct 20;437(7062):1173-8.
Specification
Aliases PS1TP5BP1; ACTB
Entrez GeneID 60
Swissprot P60709
clone 8H10D10
WB Predicted band size 42kDa
Host/Isotype Mouse IgG2b
Antibody Type Loading Control Antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human,Mouse,Hamster,Rat,Monkey,Dog
Immunogen Synthetic peptide corresponding to amino-terminal residues of human beta-Actin, conjugated to KLH.
Formulation Ascitic fluid containing 0.03% sodium azide.
Application
WB 1/500 - 1/2000
ICC 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000