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Immunofluorescence analysis of NIH/3T3 cells using EGF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Mouse Monoclonal Antibody to EGF

  • 货号:

    30015
  • 别名:

    URG; HOMG4; EGF
  • 应用:

    ICC
  • 反应种属:

    Human
  • 规格:

  • 数量:

    -
    +
  • 说明书:

    目录价¥2180

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Mouse Monoclonal Antibody to EGF

Description
Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.
References
1. Biochem J. 1992 Dec 1;288 ( Pt 2):395-405.
2. Oncogene. 2000 Mar 16;19(12):1509-18.
3. Nature. 2002 Mar 14;416(6877):183-7.
4. Radiat Res. 2003 Apr;159(4):439-52.
Specification
Aliases URG; HOMG4; EGF
Entrez GeneID 1950
Swissprot P01133
clone 4E11
WB Predicted band size 134kDa
Host/Isotype Mouse IgG1
Antibody Type Primary antibody
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen Purified recombinant fragment of human EGF expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Application
ICC 1/200 - 1/1000
ELISA 1/10000

For Reseach Only

Application Key:WB - Western Blot | IHC - Immunohistochemistry | ICC - Immunocytochemistry | FCM - Flow Cytometry | ELISA - Enzyme-linked Immunosorbent Assay | IP - Immunoprecipitation

#30015